Molecular Characterization of Fusion and Glycoprotein Genes of RSV Genotype a in Infants Suffering From Influenza Like Symptoms in Iraq

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Author(s)   Zaineb M. Naji | Maha D. Abbas | Hassan M. Naif | Hayfa H. Hassani | Faisel G. Alhamdani
Pages 537-544
Volume 5
Issue 11
Date November, 2016
Keywords RSV, Fusion Gene, Glycoprotein Gene, RT-PCR, Nested PCR
Abstract

Respiratory syncytial virus (RSV) is one of the most common respiratory viruses that causes bronchiolitis in infant all over the world. Differentiation of the contiguous genotype A and B of this virus in children less than five years old with influenza like illness (ILI) was carried out by real-time RT-PCR targeting the Fusion (F) and Glycoprotein (G) genes. Results showed that 31% of children with ILI were infected with RSV type A while no genotype B was found in any of the patients tested. Targeting partial sequence of F gene of RSV using nested RT-PCR revealed positive amplicons of 90 bp in twenty specimens. Similarly, targeting a partial sequence of the G gene of RSV revealed positive amplicons of 391bp for two isolates only in the second round of amplification. Phylogenetic analyses were conducted for the partial sequences of F and G genes type a using MEGA 6. Results showed that strain A2 was found among 61% of specimens tested for F gene. Sequencing and phylogenetic tree analyses of the G gene showed similarity to the two novel genotype of RSV type A (GA2 and ON1) circulating in children under five years old in Iraq.

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