Resistance against Glyphosate and 2-4-D in the Indigenous Strain of A. Tumefaciens “SDB0012” And Its’ Association with Oxidase Inhibition

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Author(s) Mohamed T. Yousif | Salah A. Mustafa | Aziza M. Adam | Yousif Assad
Pages 1-7
Volume 5
Issue 1
Date January, 2016
Keywords Glyphosate Herbicide Resistance Agrobacterium Oxidase Gene Pentrance
Abstract

This study was conducted at University of Gezira, Sudan; The National Institute for Promotion of Horticultural Exports and Faculty of Engineering and Technology in 2008-09. It aimed at examining resistance of the indigenous strain of A. tumefaciens “SDB0012” to application of herbicides and role of oxidase in such resistance. Pendimethalin, Glyphosate and 2-4-D were used at dilution rates of 1:200 and 1:1 of herbicide: distilled water. Only Glyphosate and 2-4-D were further investigated at dilution rates of 2:1 and 3:1. Cyanide was used as an oxidase inhibitor at dilution rates of 0.02, 0.2, 0.5 and 2.0 mg/l. It was used to inhibit oxidase activity in the bacterial suspension and on potato discs treated with the bacterium only and with herbicides. Results concluded that the bacterium “SDB0012” was sensitive to Pendimethalin at low concentrations of 1:200 and 1:1. Further investigations concluded that the bacterium resisted Glyphosate better than 2-4-D at dilution rates of 2:1 and 3:1 and that resistance to both herbicides was highly stable. Results also indicated that resistance to Glyphosate and 2-4-D was not associated and independent. Resistance to both herbicides was found positively affected by time of incubation. Results also showed that the intensity of purple color, as an indicator for oxidase, reduced as the concentration of cyanide increased from 0.02 to 2.0 mg/l. This result indicated that oxidase had no effect on resistance of the bacterium against Glyphosate and 2-4-D. Therefore, it was suggested that this resistance might be due to effects of other independent gene(s).

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