Stability-Indicating RP-HPLC Method Development for Simultaneous Determination and Estimation of Bendroflumethiazide and Nadolol in Raw and Tablet Formulation

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Author(s) Gunasekar Manoharan
Pages 589-596
Volume 5
Issue 12
Date December, 2016
Keywords Calcium Carbide Residue, Hydrated Lime, Stabilization Agent

A sensitive, feasible RP-HPLC method has developed and validated for the analysis of Bendroflumethiazide and Nadolol in raw and tablet formulation. Successful separation of drugs products is developed on a C (18) column reversed-phase using and using mobile phase composition of Acetonitrile: Methanol: Water (30:20:50 v/v/v). The flow rate was adjusted to 1 mL/minute and the absorption maxima were observed at 250 nm utilizing Shimadzu SPD-20A Prominence UV-Vis detector. Good linearity was obtained in the range of 2-10 μg/ ml, 5-25 μg/ml, for Bendroflumethiazide, Nadolol respectively. The HPLC, tablet formulation assay shows percentage purity ranging from 100.12 to 99.94% for Bendroflumethiazide, 100.97 to 99.73%for Nadolol. The mean percentage purity is 100.01% and 100.07% for Bendroflumethiazide and Nadolol respectively. The chromatographic retention time of Bendroflumethiazide and Nadolol was found to be 2.2 and 5.7 minutes respectively. The tailing factor was 0.779 and 0.980 for Bendroflumethiazide and Nadolol respectively. The developed method validated according to the ICH guidelines. The method was found to be applicable for determination and validation of Bendroflumethiazide and Nadolol in combined tablet form.

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