Isolation, identification and characterization of soil Actinobacteria from Jazan, Saudi Arabia

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Author(s) Ranaa Mujamammi | Islem Abid | Muneera Al-Othman | Muneera D.F. Alkahtani
Pages 767-775
Volume 3
Issue 12
Date December, 2014
Keywords Actinobacteria, Jazan, Scan Electron Microscope, Physiological analysis, Biochemical analysis
Abstract

Actinobacteria are a gram-positive, filamentous subgroup of bacteria most known for antibiotic production. The exploration of new habitats with unusual environment and poorly explored areas of the world has become important and useful to the discovery of novel compounds and actinobacteria. In this study, we have isolated actinobacteria species from 5 different soil samples from different locations (Sabya, Baish Samtah, Alddarb and Abu Arish) in Jazan, Saudi Arabia. The soils were characterized for physicochemical analysis. The comparisons of the actinobacteria abundance with the physicochemical characteristics of these soils were also performed. There were no differences in the soil textures (sandy loam) and organic matter values from all the regions. In general, the physical and chemical characteristics for all the soils analyzed were similar. A total of 270 actinobacteria strains were isolated. All purified isolates grew on yeast starch agar media showing morphology of typical Streptomyces. Total bacterial counts were compared between the five collection sites of soil samples. Alddarb exhibited higher microbial counts (10.48 log CFU/g) compared to Baish and Samtah with a minimum of (10.31 log CFU/g). 6 isolates designated: JS3, JS4, JS6, JD7, JA8 and JA10, were selected with effective in terms of colony color and morphology and were subject to cultural, morphological, physiological and biochemical analysis. The present data suggest that the number and diversity of actinobacteria in desertic soils represent a vast unexplored resource for the biotechnology of bioactives production.

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