Culture Conditions for the Production of Tannase from Trichoderma harzianum MTCC 10841

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Author(s) Hina Iqbal | Ashima Kapoor
Pages 584-595
Volume 1
Issue 11
Date November, 2012
Keywords Amla fruits, Amaltash leaves, tannase production, Trichoderma harzianum

A total of 36 fungal strains were isolated from the different soil samples collected around the tannin-rich plants. The isolated cultures were screened for their tannase producing capability by observing the zone of hydrolysis on tannic acid agar plates. Among the twenty two fungal strains selected as tannase producers, the strain T16 produced highest (16.36 U/ml) tannase activity. Hence, it was selected for further study and identified as Trichoderma harzianum MTCC 10841. To enhance the production level of the enzyme different culture conditions were optimized and observed that optimum temperature and pH for tannase production was 30oC and 5.5 respectively. Maximum growth and enzyme production was recorded after 96 hrs of incubation period in the medium containing 1% tannic acid. Among the different natural substrates tested as carbon source, amla fruit enhanced the tannase production by almost two fold compared to pure tannic acid used as a substrate. Pomegranate rind, jamun bark and amaltash leaves also supported tannase production appreciably. Malt extract (2%) with NH4Cl (0.2%) was found to the best nitrogen source. Among the additives, metal ions Ca++, Na+, Mg++, Mn++ and Zn++ did not affect enzyme production. However, metal ions like K+, Cu++, Co++, Fe+++ and the various detergents tested, inhibited the production of tannase. The optimization of culture conditions enhanced the production level of tannase (32.2 U/ml) by two-fold. The tannase production was successfully scaled up to 1.5L at laboratory level.

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