Indentification of Potent Inhibitors of Chilo partellus (Swinhoe) (Lepidoptera: Pyralidae) Gut Proteinase from Plant Gum PIs

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Author(s) Balaji M. Pancha | Manvendra S.Kachole
Pages 662-670
Volume 1
Issue 12
Date December, 2012
Keywords Chilo partellus (Swinhoe) (Lepidoptera: Pyralidae) insects; CPGPs; Host and non-host PIs; BApNA; GXCPS

We report on the efficacy of proteinase inhibitors (PIs) from six host plants Zea mays, m35 sorghum, Sorghum bicolour, Saccharum spp., Setaria italia and Pennisetum glaucum (L) stem tissue and five non-host Acacia nilotica, Acacia leucophloea, Azardirachta indica, Mangifera indica and Terminalia spp. plant gum extracts PIs, in retarding the growth of Chilo partellus (Swinhoe) (Lepidoptera: Pyralidae) larvae, a devastating pest of important crop plants. Enzyme assays and electrophoretic analysis of interaction of C.partellus gut proteinases (CPGPs) with PIs revealed that non-host PIs inhibited CPGPs activity efficiently whereas host PIs were ineffective. In the electrophoretic assay, trypsin inhibitor activity bands were detected in all of the host and non-host plants, but CPGPs inhibitor activity bands were present only in non-host plants. C. partellus larvae reared on a diet containing non-host PIs showed growth retardation, reduction in total and trypsin like proteinase activity. In present study potent PIs identified against C. partellus linsects.

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